And on Thursday the club confirmed Kane had sustained "significant damage".
"Following assessment over the past two days, we can confirm that Harry Kane has sustained a significant lateral ligament injury to his left ankle following a challenge in our Champions League quarter-final, first leg win against Manchester City," read a statement on the club's official website.
The news comes as a huge blow for the 135,000+ managers who transferred Kane in for his home match against Huddersfield Town on Saturday lunchtime.
He has been sold by over 68,000 managers in the last 24 hours, as his 15.9 per cent FPL owners reassess their options ahead of Friday's 19:00 BST deadline.
But if you are one of those yet to replace the two-time Golden Boot winner, who are the best alternatives to step in?
Sergio Aguero (£11.7m) has been the most popular replacement, brought in by 28,000+ Kane owners.
The Man City forward has six matches in the final five Gameweeks, including a Double Gameweek 35.
Vardy is the most in-form striker in FPL, having scored seven goals and 55 points in his last six appearances.
But there is another option for FPL managers.
Rather than spend Kane funds in attack, it may instead be worth investing in Spurs' midfielders.
That is because even without Kane they have a favourable schedule which lifts the potential of their key attackers.
Four of their remaining six fixtures are at home and they have a Double Gameweek 35, when they face Man City and Brighton & Hove Albion.
The South Korean is the most-bought player in the Gameweek, earning more than 160,000 transfers in.
Eriksen was Spurs’ top performer in Double Gameweek 32, returning 20 points.
Both are riskier picks but have the fixtures to bring in big points if they can secure places in the starting line-up.
Part 2: GW34 Captains: Son the leading contender
Part 3: GW34 Captains: Vardy to challenge again
Part 4: FPL Show: Leave out Liverpool stars at your peril
Part 5: GW34 Scout Selection: Rashford to deliver
Part 6: FPL Experts: Ryan the No 1 Double Gameweek choice
Part 7: GW34 Team news: Gross returns to managers' plans